Fourier phase microscopy with white light
نویسندگان
چکیده
منابع مشابه
Fourier phase microscopy with white light.
Laser-based Fourier phase microscopy (FPM) works on the principle of decomposition of an image field in two spatial components that can be controllably shifted in phase with respect to each other. However, due to the coherent illumination, the contrast in phase images is degraded by speckles. In this paper we present FPM with spatially coherent white light (wFPM), which offers high spatial phas...
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We present white light diffraction phase microscopy (wDPM) as a quantitative phase imaging method that combines the single shot measurement benefit associated with off-axis methods, high temporal phase stability associated with common path geometries, and high spatial phase sensitivity due to the white light illumination. We propose a spatiotemporal filtering method that pushes the limit of the...
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Quantitative phase imaging (QPI) techniques are very advantageous compared to the qualitative phase imaging techniques like bright field microscopy, phase contrast microscopy, differential interference contrast microscopy due to measurement of phase information quantitatively. In QPI techniques, there is no need of exogenous contrast agents to stain or tag the specimen. White light diffraction ...
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We demonstrate the first reflection-based epi-illumination diffraction phase microscope with white light (epi-wDPM). The epi-wDPM system combines the off-axis, common-path, and white light approaches, in a reflection geometry enabling sub-nanometer spatial and temporal noise levels, while providing single-shot acquisition for opaque samples. We verified the epi-wDPM results by measuring control...
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In off-axis interferometry, we usually have to deal with the unwrapping process, which is very computationally intensive and prevents us from real time phase reconstruction. The wrapping problem usually occurs when imaging thick objects, which introduce phase shifts of more than 2π radians. However, in off-axis interferometry, the nonzero angle of interference of the two beams creates a ramp in...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2013
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.4.001434